Lady Macbeth's Stain a Piano Work by American Composer/Pianist David Hart

Despite his success, Macbeth remains uneasy about the prophecy about Banquo. So Macbeth invites him to a royal banquet and discovers that Banquo and his young son, Fleance, will be riding out that night. He hires two men to kill them. A third murderer appears mysteriously in the park before the murder. While the assassins kill Banquo, Fleance escapes. At the banquet, Banquo's ghost enters and sits in Macbeth's place. Only Macbeth can see the spectre; the rest panic at the sight of Macbeth raging at an empty chair, until a desperate Lady Macbeth orders them to leave.

Macbeth, disturbed, goes to the Witches once more. They conjure up three spirits with three further warnings and prophecies, which tell him to "beware Macduff", but also that "none of woman born shall harm Macbeth" and he will "never vanquish'd be until Great Birnam Wood to high Dunsinane Hill shall come against him". Since Macduff is in exile in England, Macbeth assumes that he is safe; so he puts to death everyone in Macduff's castle, including Macduff's wife and their young children.

Lady Macbeth becomes wracked with guilt from the crimes she and her husband have committed. In a famous scene, she sleepwalks and tries to wash imaginary bloodstains from her hands, all the while speaking of the terrible things she knows.